Auramine-rhodamine stain

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File:Cryptosporidium parvum auramine-rhodamine labeled.jpg
Oocysts of Cryptosporidium parvum stained with the fluorescent auramine-rhodamine stain.

The auramine-rhodamine stain (AR), also known as the Truant auramine-rhodamine stain, is a histological technique used to visualize acid-fast bacilli using fluorescence microscopy, notably species in the Mycobacterium genus.[1] Acid-fast organisms display a reddish-yellow fluorescence.[2] Although the auramine-rhodamine stain is not as specific for acid-fast organisms (i.e. Mycobacterium tuberculosis or Nocardia) as the Ziehl-Neelsen stain, it is more affordable and more sensitive, therefore it is often utilized as a screening tool.

AR stain is a mixture of Auramine O and Rhodamine B.

See also

References

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