Citrus taxonomy

Various citrus types in cross section. Some of them are hybrids between two or more original species.
The investigation of this matter and the botanical classification of the species, varieties and cultivars belonging to the genus citrus, is called "citrus taxonomy".

Citrus taxonomy refers to the botanical classification of the species, varieties, cultivars, and graft hybrids within the genus Citrus, subgenus Papeda, and related genera, found in cultivation and in the wild.

Citrus taxonomy is complex. Cultivated citrus are derived from various forms of old citrus species found in the wild. Some are only selections of the original wild types, while others are hybrids between two or more ancestors. Citrus plants hybridize easily between species with completely different morphologies, and similar-looking citrus fruits may have quite different ancestries.

Most hybrids express different ancestral traits when planted from seeds (F2 hybrids) and can continue a stable lineage only through vegetative propagation. Others do reproduce true to type via nucellar seeds in a process called apomixis. Many different-looking varieties are nearly genetically identical, and differ only by a bud mutation. Some differ only in disease resistance.

Clear genetic lineages are very important for breeding improved cultivars.

Genetic history

Citrus fruits clustered by genetic similarity (PCA of SNP diversity). Citrus micrantha (top right) is a papeda.

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Hybrids are expected to plot between their parents. ML: ‘Mexican’ lime; A: ‘Alemow’; V: ‘Volkamer’ lemon; M: ‘Meyer’ lemon; L: Regular and ‘Sweet’ lemons; B: Bergamot; H: Haploid clementine; C: Clementines; S: Sour oranges; O: Sweet oranges; G: Grapefruits.

Interbreeding seems possible between all citrus plants, and between citrus plants and some plants which may or may not be categorized as citrus. The ability of citrus hybrids to self-pollinate and to reproduce sexually also helps create new varieties.

The four core ancestral citrus taxa are citron (C. medica), pummelo (C. maxima), mandarine (C. reticulata), and papeda (C. micrantha).

These taxa all interbreed freely, despite being quite genetically distinct. They probably arose through allopatric speciation, with citrons evolving in northern Indochina, pummelos in the Malay Archipelago, and mandarines in Vietnam, southern China, and Japan.

The hybrids of these four taxa include familiar citrus fruits like oranges, grapefruit, lemons, limes, and some tangerines. In many cases, these crops are propagated asexually, and lose their characteristic traits if bred. However, some of these hybrids have interbred with one another and with the original taxa, making the citrus family tree a complicated network.

There are also groups that interbreed with the four core taxa, but which have not historically been categorized as citrus. The trifoliate orange and kumquats do not naturally interbreed with the four undisputed citrus taxa due to different flowering times, but hybrids (such as the citrange and calamondin) exist. Australian limes and Clymenia are native to Australia and Papua New Guinea, so they did not naturally interbreed with the four core taxa; but they have been crossbred with mandarins and calamondins by modern breeders.

Humans have deliberately bred new citrus fruits by propagating wild-found seedlings (e.g. clementines), creating or selecting mutations of hybrids, (e.g. Meyer lemon), and crossing different varieties (e.g. 'Australian Sunrise', a finger lime and calamondin cross).

Genetic analysis is starting to make sense of this complex phylogeny. Two citrus fruits have had their full genomes sequenced (sweet orange and clementines). Many different phylogenies for the non-hybrid citrus have been proposed, and taxonomic terminology is not yet settled.

Citrus naming systems

See also: Botanical name

Because citrus taxonomy is still understudied, there are many systems of classification that very often contradict each other. There are two main complete systems, that of Tanaka and that of Swingle; however, many citrus types were identified and named by individual taxonomists.

The basis of the "Tanaka system" is to provide a separate name for each cultivar, regardless of whether it is pure or a hybrid of two or more species or varieties. The "Swingle system" works differently, in that it first divides the genus citrus into species, secondly divides it further into varieties, and lastly divides it again into cultivars or hybrids. The Swingle system is generally followed today with much modification; however, there are still huge differences in nomenclature between countries and even individual scientists.

Common name confusions

See also: Folk taxonomy

The Lumia

The Limetta

The same common names may be given to different citrus hybrids or mutations. There are numerous examples:

Sweet lemon

Sweet lemon or sweet lime may refer to a citrus fruit that is identical to a common lemon or lime, but is lacking the common acidic taste usually found in lemons or limes. Those names are however applied to a number of fruits from different origins, for example:

1. The lumia from the Mediterranean basin, probably Italy, is a big dry citron-like citrus that is pear shaped and not necessarily sweet.
2. The Persian sweet lime, also known as the limetta, is small and round like a common lime with sweet juice.
3. The Palestinian sweet lime from India is mainly used as a rootstock.

The following is from the Purdue University Website:

The sweet lime, Citrus limettioides Tan. (syn. C. lumia Risso et Poit.), is often confused with the sweet lemon, C. limetta Tan., (q.v. under LEMON) which, in certain areas, is referred to as "sweet lime". In some of the literature, it is impossible to tell which fruit is under discussion.

The following is from the The Citrus Industry Volume I:

The Indian sweet lime is the mitha nimbu (numerous modifications and other local names) of India, the limûn helou or succari of Egypt, and the Palestine sweet lime (to distinguish it from the Millsweet and Tunisian limettas, commonly called sweet limes).

The common oranges as well as the grapefruits are hybrids between the mandarin and the pummelo.

Genetically, one study showed that the Persian sweet lemon clusters with the pummelo, mandarin orange and common orange groups, and not with the citrons, limes and lemons group. Another study showed that most lemons are from one common ancestor, some are pure citrons or nearly so, and some are citron hybrids with a differing other parent.

Oranges

Mandarin orange is a true species, it is one of the progenitors of most cultivated citrus.

Trifoliate orange belongs to the genus poncirus, according to Swingle.

Another good example of citrus fruits are oranges. Not all the fruits that are called by the name "orange" share genetic affiliation. The common sweet orange and sour orange genetics are like those of the grapefruit, a cross between a pommelo and a mandarin orange. They are all intermediate between the two ancestors in size, flavor and shape. The above-mentioned oranges have the orange color of the mandarin orange in their outer peels and segments, and are easier to peel than the grapefruits, which are more akin to the pommelo ancestor and so probably a cross between the sweet orange and the pommelo.

There is genetic diversity between the mandarin oranges, and many of them are actually hybrids.

The trifoliate orange is a cold hardy plant distinguishable by its leaves with three leaflets. It is the only member of the Poncirus genus (disputed), but is close enough to the Citrus genus to be used as a rootstock. The Indian wild orange is, according to some research, one of the ancestors of today's cultivated citrus fruits. The bergamot orange is likely to be a hybrid of Citrus limetta and Citrus aurantium.

Citron varieties

Two varieties of citron, etrog and fingered, are both citrons but are extremely distinct-looking.

3 more varieties of Citrus medica that are all true and pure citrons.

Citron has many true varieties that are extremely distinct looking. Some of the fingered varieties are used in a buddhist offering and some of the common varieties are used for the Jewish ritual etrog, and there is also a variety of citron called etrog.

While in Modern Hebrew etrog is the name for citron of any variety or form, whether kosher for the ritual or not, its English usage applies only to those varieties and specimens used as one of the Four Species. The various Jewish rites utilize different varieties, according to their tradition or the decision of their respective Posek. The Mountain citron, however, has no relation to the true citron and belongs to the citrus subg. Papeda.

Citron in general is believed to be a very pure ancestral species. It is usually fertilized by self-pollination, and is therefore generally a male parent of any citrus hybrid, rather than a female one. Many citron varieties were proven to be pure despite their morphological differences; however, the florentine citron is probably of hybrid origin.

Sweet and sour

Principally sweet and sour varieties are found in true species such as in citrons, as well as in hybrid lemons and oranges. The Moroccan professor Henri Chapot discovered that the acidity in the more common citrons or lemons is represented by red on the inner coat of seeds specifically on the chalazal spot, violet pigmentation on the outer side of the flower blossom, and also by the new buds that are reddish-purplish. The acidless varieties of citrus are completely lacking the red color in all the mentioned spots. This designation was cited by Herbert John Webber and Leon Dexter Batchelor the editors of the fundamental treatise on citrus, namely The Citrus Industry, which was published by the University of California, Riverside in 1967.

Citrus relatives

Another issue of controversy is whether the citrus-related genera are to be included in the genus or not.

Papeda

Swingle coined the Citrus subg. Papeda to separate its members from the more edible citrus. Papedas also differ from regular citrus in that its stamens grow separately, not united at the base.

Fortunella & Citrofortunella

Carl Peter Thunberg originally classified the kumquats as Citrus japonica in his 1784 book, Flora Japonica. In 1915, Walter T. Swingle reclassified them in a segregate genus, Fortunella, named in honor of Robert Fortune. Seven species of Fortunella have generally been recognized—F. japonica, F. margarita, F. crassifolia, F. hindsii, F. obovata and F. polyandra, as well as the recently described F. bawangica. Since the kumquat is a cold hardy species, there are many hybrids between common citrus members and the kumquat (most popularly the calamondin that occurred naturally). Those hybrids are not citrus hybrids, according to Swingle, but reside in a separate hybrid genus which he called × Citrofortunella.

Subsequent study of the many commercial lineages revealed such complexity that the genera could not be separated. Consequently, in accordance with the International Code of Nomenclature for algae, fungi, and plants, the correct genus name reverted to Citrus. The Flora of China returns the kumquat to Citrus and combines the species into the single species as Citrus japonica, and today the Fortunella and Citrofortunella are nothing else but regular citrus.

Poncirus and Citrocirus

According to the Swingle system, the trifoliate orange, a cold hardy plant that is commonly used as a citrus rootstock, is not included in the genus Citrus, but in a related genus, Poncirus. Therefore, the citrange, which is a hybrid between the trifoliate and the sweet orange, is placed into a hybrid genus called Citrocirus (not a valid botanical name). However, molecular investigation suggests Poncirus should be equivocally included in the genus Citrus.

Reclassification of Microcitrus and Eremocitrus

The desert lime, formerly Eremocitrus, but now Citrus glauca, hangs from a branch.

The edible Australian limes are starting to be domesticated and commercially grown. Recent molecular research suggests that although Swingle placed them in the separate genera Microcitrus and Eremocitrus, they should also be included in the genus Citrus.

Triphasia and Clymenia

Two additional genera, Triphasia and Clymenia, are likewise very closely related to the Citrus genus and bear hesperidium fruits, but they are not usually considered part of it. At least one, Clymenia, will hybridize with kumquats and some limes.

Wild lime

The wild lime—native to southern Florida and Texas in the United States, Mexico, Central America, the Caribbean, and South America as far south as Paraguay—does not belong in the Citrus genus. It is part of another genus in the Rutaceae family, Zanthoxylum (which also includes Sichuan pepper), and was classified as Zanthoxylum fagara by Charles Sprague Sargent.

International taxonomy

The International Association for Plant Taxonomy was founded in 1950 to resolve the taxonomic problem of all the plants. They hold meetings every five years to review the updated information and try to reach consensus. The current president is David Mabberley.

See also

• Plants portal
• Agriculture and Agronomy portal
• Plant taxonomy

References

Books

• Citrus: The Genus Citrus
• The Citrus Industry (book) Online

External links

• USDA Citrus Genome Database
• USDA Classification
• Genetic Diversity in Citrus
• A classification for edible Citrus (Rutaceae)
• Citrus Pages - a comprehensive article on citrus taxonomy
• Burkill, I. H. (1931). An enumeration of the species of Paramignya, Atalantia and Citrus, found in Malaya. Gard. Bull. Straits Settlem. 5: 212–220.
• Mabberley, D. J. (1998). Australian Citreae with notes on other Aurantioideae (Rutaceae). Telopea 7 (4): 333–344. Available online (pdf).
• Fruits of warm climates
• Fortunella crassifolia Swingle - Fruits and Seeds Flavon's Wild herb and Alpine plants
• International Code of Botanical Nomenclature (Tokyo Code)

Molecular classification

• DNA ANALYSIS AS A TOOL FOR CITRUS RETICULATA ADULTERATION DETECTION AND VARIETY IDENTIFICATION IN COMMERCIAL ORANGE JUICES
• DNA Amplified Fingerprinting, A Useful Tool for Determination of Genetic Origin and Diversity Analysis in Citrus