Factor IX

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Coagulation factor IX
PDB 1pfx EBI.jpg
PDB rendering based on 1pfx.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols F9 ; FIX; HEMB; P19; PTC; THPH8
External IDs OMIM300746 MGI88384 HomoloGene106 ChEMBL: 2016 GeneCards: F9 Gene
EC number 3.4.21.22
RNA expression pattern
PBB GE F9 207218 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 2158 14071
Ensembl ENSG00000101981 ENSMUSG00000031138
UniProt P00740 P16294
RefSeq (mRNA) NM_000133 NM_001305797
RefSeq (protein) NP_000124 NP_001292726
Location (UCSC) Chr X:
139.53 – 139.56 Mb
Chr X:
60 – 60.03 Mb
PubMed search [1] [2]

Factor IX (or Christmas factor) (EC 3.4.21.22) is one of the serine proteases of the coagulation system; it belongs to peptidase family S1. Deficiency of this protein causes hemophilia B. It was discovered in 1952 after a young boy named Stephen Christmas was found to be lacking this exact factor, leading to hemophilia.[1]

It is on the WHO Model List of Essential Medicines, the most important medications needed in a basic health system.[2]

Physiology

The blood coagulation and Protein C pathway.

Factor IX is produced as a zymogen, an inactive precursor. It is processed to remove the signal peptide, glycosylated and then cleaved by factor XIa (of the contact pathway) or factor VIIa (of the tissue factor pathway) to produce a two-chain form where the chains are linked by a disulfide bridge.[3][4] When activated into factor IXa, in the presence of Ca2+, membrane phospholipids, and a Factor VIII cofactor, it hydrolyses one arginine-isoleucine bond in factor X to form factor Xa.

Factor IX is inhibited by antithrombin.[3]

Factor IX expression increases with age in humans and mice. In mouse models mutations within the promoter region of factor IX have an age-dependent phenotype.[5]

Domain architecture

Factors VII, IX, and X all play key roles in blood coagulation and also share a common domain architecture.[6] The factor IX protein is composed of four protein domains: the Gla domain, two tandem copies of the EGF domain and a C-terminal trypsin-like peptidase domain which carries out the catalytic cleavage.

Human factor IX protein domain architecture, where each protein domain is represented by a coloured box

The N-terminal EGF domain has been shown to at least in part be responsible for binding tissue factor.[6] Wilkinson et al. conclude that residues 88 to 109 of the second EGF domain mediate binding to platelets and assembly of the factor X activating complex.[7]

The structures of all four domains have been solved. A structure of the two EGF domains and the trypsin-like domain was determined for the pig protein.[8] The structure of the Gla domain, which is responsible for Ca(II)-dependent phospholipid binding, was also determined by NMR.[9]

Several structures of 'super active' mutants have been solved,[10] which reveal the nature of factor IX activation by other proteins in the clotting cascade.

Genetics

The gene for factor IX is located on the X chromosome (Xq27.1-q27.2) and is therefore X-linked recessive: mutations in this gene affect males much more frequently than females. It was first cloned in 1982 by Kotoku Kurachi and Earl Davie.[11]

Polly, a transgenic cloned Poll Dorset sheep carrying the gene for factor IX, was produced by Dr Ian Wilmut at the Roslin Institute in 1997.[12]

Role in disease

Deficiency of factor IX causes Christmas disease (hemophilia B).[1] Over 100 mutations of factor IX have been described; some cause no symptoms, but many lead to a significant bleeding disorder. The original Christmas disease mutation was identified by sequencing of Christmas' DNA, revealing a mutation which changed a cysteine to a serine.[13] Recombinant factor IX is used to treat Christmas disease, and is commercially available as BeneFIX[14] and Alprolix.[15] Some rare mutations of factor IX result in elevated clotting activity, and can result in clotting diseases, such as deep vein thrombosis.[16]

Factor IX deficiency is treated by injection of purified factor IX produced through cloning in various animal or animal cell vectors. Tranexamic acid may be of value in patients undergoing surgery who have inherited factor IX deficiency in order to reduce the perioperative risk of bleeding.[17]

A list of all the mutations in Factor IX is compiled and maintained at the Factor IX mutation database[18] maintained at the University College London.

References

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Further reading

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External links