Glutathione reductase

Glutathione reductase
250px PDB rendering based on 1GRE.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1ALG, 1BWC, 1DNC, 1GRA, 1GRB, 1GRE, 1GRF, 1GRG, 1GRH, 1GRT, 1GSN, 1K4Q, 1XAN, 2AAQ, 2GH5, 2GRT, 3DJG, 3DJJ, 3DK4, 3DK8, 3DK9, 3GRS, 3GRT, 3SQP, 4GR1, 4GRT, 5GRT
Identifiers
Symbols	GSR ; HEL-75
External IDs	OMIM: 138300 MGI: 95804 HomoloGene: 531 ChEMBL: 2755 GeneCards: GSR Gene
EC number	1.8.1.7
Gene ontology Molecular function • glutathione-disulfide reductase activity • electron carrier activity • flavin adenine dinucleotide binding • NADP binding Cellular component • mitochondrial matrix • cytosol • external side of plasma membrane • extracellular exosome Biological process • response to reactive oxygen species • transcription initiation from RNA polymerase II promoter • glutathione metabolic process • gene expression • nucleobase-containing small molecule interconversion • small molecule metabolic process • cell redox homeostasis • nucleobase-containing small molecule metabolic process • oxidation-reduction process Sources: Amigo / QuickGO
RNA expression pattern
File:PBB GE GSR 205770 at tn.png
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	2936	14782
Ensembl	ENSG00000104687	ENSMUSG00000031584
UniProt	P00390	P47791
RefSeq (mRNA)	NM_000637	NM_010344
RefSeq (protein)	NP_000628	NP_034474
Location (UCSC)	Chr 8: 30.68 – 30.73 Mb	Chr 8: 33.65 – 33.7 Mb
PubMed search	[1]	[2]

Glutathione reductase (GR) also known as glutathione-disulfide reductase (GSR) is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase (EC 1.8.1.7) catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. Glutathione reductase functions as dimeric disulfide oxidoreductase and utilizes an FAD prosthetic group and NADPH to reduce one molar equivalent of GSSG to two molar equivalents of GSH:

File:GSR Scheme 1.PNG

General reaction catalyzed by glutathione reductase

The glutathione reductase is conserved between all kingdoms. In bacteria, yeasts, and animals, one glutathione reductase gene is found; however, in plant genomes, two GR genes are encoded. Drosophila and Trypanosomes do not have any GR at all. In these organisms, glutathione reduction is performed by either the thioredoxin or the trypanothione system, respectively.

Function

glutathione-disulfide reductase
File:GSR Model Figure.PNG Human GSR with bound glutathione and FADH
Identifiers
EC number	1.8.1.7
CAS number	Template:CAS
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

Glutathione plays a key role in maintaining proper function and preventing oxidative stress in human cells. It can act as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces hydrogen peroxide (H2O2), a dangerously reactive species within the cell. In addition, it plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, participates in transport, and regenerates antioxidants such and Vitamins E and C to their reactive forms. The ratio of GSSH/GSH present in the cell is a key factor in properly maintaining the oxidative balance of the cell, that is, it is critical that the cell maintains high levels of the reduced glutathione and a low level of the oxidized Glutathione disulfide. This narrow balance is maintained by glutathione reductase, which catalyzes the reduction of GSSG to GSH.

File:Gluthathione Reductase Graphic 7.jpg

Reduced glutathione reductase, glutathione peroxidase and glutathione interact to reduce hydrogen peroxide to water, in order to protect the cell from oxidative damage.

Structure

Glutathione reductase from human erythrocytes is a homodimer consisting of 52Kd monomers, each containing 3 domains. GR exhibits single sheet, double layered topology where an anti-parallel beta-sheet is largely exposed to the solvent on one face while being covered by random coils on the other face. This includes and NADPH-binding Domain, FAD-binding domain(s) and a dimerization domain. Each monomer contains 478 residues and one FAD molecule. GR is a thermostable protein, retaining function up to 65^oC.

Reaction mechanism

File:GSR Reaction Figure.PNG

Graphical representation of overall reaction catalyzed by GR

File:GSR Catalytic Cycle.PNG

GR catalytic cycle

Steps:

1	NADPH binding to the oxidized enzyme
2	Reduction of FAD to FADH− anion by NADPH
3	Reduced FADH− anion collapses into a charge relay complex and reduces Cys58-Cys63 disulfide
4	Oxidized Glutathione disulfide binds to the reduced enzyme and forms a mixed disulfide with Cys58 and releases one reduced glutathione
5	Cys63 attacks the mixed disulfide on Cys58 to release a reduced glutathione and reform the redox active disulfide

Reductive half

The action of GR proceeds through two distinct half reactions, a reductive half mechanism followed by an oxidative half. In the first half, NADPH reduces FAD present in GSR to produce a transient FADH⁻ anion. This anion then quickly breaks a disulfide bond of Cys₅₈ - Cys₆₃, forming a short lived covalent bond a stable charge-transfer complex between the flavin and Cys₆₃. The now oxidized NADP+ is released and is subsequently replaced by a new molecule of NADPH. This is the end of the so-called reductive half of the mechanism.

Oxidative half

In the oxidative half of the meachanism, Cys₆₃ nucleophilically attacks the nearest sulfide unit in the GSSG molecule (promoted by His₄₆₇), which creates a mixed disulfide bond (GS-Cys₅₈) and a GS⁻ anion. His₄₆₇ of GSR then protonates the GS- anion to release the first molecule of GSH. Next, Cys₅₈ nucleophilically attacks the sulfide of Cys₅₈, releasing a GS⁻ anion, which, in turn, picks up a solvent proton and is released from the enzyme, thereby creating the second GSH. So, for every GSSG and NADPH, two reduced GSH molecules are gained, which can again act as antioxidants scavenging reactive oxygen species in the cell.

Inhibition

In vitro, glutathione reductase is inhibited by low concentrations of sodium arsenite and methylated arensate metabolites, but in vivo, significant Glutathione Reductase inhibition by sodium arsenate has only been at 10 mg/kg/day. Glutathione reductase is also inhibited by some flavanoids, a class of pigments produced by plants.

Clinical significance

GSH is a key cellular antioxidant and plays a major role in the phase 2 metabolic clearance of electrophilic xenobiotics. The importance of the GSH pathway and enzymes that affect this delicate balance is gaining an increased level of attention in recent years. Although glutathione reductase has been an attractive target for many pharmaceuticals, there have been no successful glutathione reductase related therapeutic compounds created to date. In particular, glutathione reductase appears to be a good target for anti-malarials, as the glutathione reductase of the malaria parasite plasmodium falciparum has a significantly different protein fold than that of mammalian glutathione reductase. By designing drugs specific to p. falciparum it may be possible to selectively induce oxidative stress in the parasite, while not affecting the host.

There are two main classes of GR targeting compounds:

1. Inhibitors of GSSG binding, or dimerization: Reactive electrophiles such as gold compounds, and fluoronaphthoquinones.
2. Drugs which use glutathione reductase to regenerate, such as redox cyclers. Two examples of these types of compounds are Methylene blue and Naphthoquinone.

Clinical trials performed in Burkina Faso have revealed mixed results when treating malaria with Naphthoquinones

In cells exposed to high levels of oxidative stress, like red blood cells, up to 10% of the glucose consumption may be directed to the pentose phosphate pathway (PPP) for production of the NADPH needed for this reaction. In the case of erythrocytes, if the PPP is non-functional, then the oxidative stress in the cell will lead to cell lysis and anemia.

Lupus is an autoimmune disorder in which patients produce an elevated quantity of antibodies that attack DNA and other cell components. In a recent study, a single nucleotide polymorphism (SNP) in the Glutathione Reductase gene was found to be highly associated with lupus in African Americans in the study. African Americans with lupus have also been shown to express less reduced glutathione in their T cells. The study's authors believe that reduced glutathione reductase activity may contribute to the increased production of reactive oxygen in African Americans with lupus.

In mice, glutathione reductase has been implicated in the oxidative burst, a component of the immune response. The oxidative burst is a defense mechanism in which neutrophils produce and release reactive oxidative species in the vicinity of bacteria or fungi to destroy the foreign cells. Glutathione Reductase deficient neutrophils were shown to produce a more transient oxidative burst in response to bacteria than neutrophils that express GR at ordinary levels. The mechanism of Glutathione Reductase in sustaining the oxidative burst is still unknown.

Deficiency

Glutathione reductase deficiency is a rare disorder in which the glutathione reductase activity is absent from erythrocytes, leukocytes or both. In one study this disorder was observed in only two cases in 15,000 tests for glutathione reductase deficiency performed over the course of 30 years. In the same study, glutathione reductase deficiency was associated with cataracts and favism in one patient and their family, and with severe unconjugated hyperbilirubinemia in another patient. It has been proposed that the glutathione redox system (of which glutathione reductase is apart) is almost exclusively responsible for the protecting of eye lens cells from hydrogen peroxide because these cells are deficient in catalase, the enzyme which catalyzes the breakdown of hydrogen peroxide, and the high rate of cataract incidence in glutathione reductase deficient individuals.

Some patients exhibit deficient levels of glutathione activity as a result of not consuming enough riboflavin in their diets. Riboflavin is a precursor for FAD, whose reduced form donates two electron to the disulfide bond which is present in the oxidized form of glutathione reductase in order to begin the enzyme's catalytic cycle. In 1999, a study found that 17.8% of males and 22.4% of females examined in Saudi Arabia suffered from low glutathione reductase activity due to riboflavin deficiency.

Connection to Favism

In Favism, patients lack glucose-6-phosphate dehydrogenase, an enzyme in their pentose phosphate pathway that reduces NADP⁺ to NADPH while catalyzing the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone. Glucose-6-phosphate dehydrogenase deficient individuals have less NADPH available for the reduction of oxidized glutathione via glutathione reductase. Thus their basal ratio of oxidized to reduced glutathione is significantly higher than that of patients who express glucose-6-phosphate dehydrogenase, normally, making them unable to effectively respond to high levels of reactive oxygen species, which cause cell lysis.

Monitoring glutathione reductase activity

The activity of glutathione reductase is used as indicator for oxidative stress. The activity can be monitored by the NADPH consumption, with absorbance at 340 nm, or the formed GSH can be visualized by Ellman's reagent. Alternatively the activity can be measured using roGFP (redox-sensitive Green Fluorescent Protein).

Glutathione reductase in plants

As it does in human cells, glutathione reductase helps to protect plant cells from reactive oxygen species. In plants, reduced glutathione participates in the glutathione-ascorbate cycle in which reduced glutathione reduces dehydroascorbate, a reactive byproduct of the reduction of hydrogen peroxide. In particular, Glutathione reductase contributes to plants' response to abiotic stress. The enzyme's activity has been shown to be modulated in response to metals, metalloids, salinity, drought, UV radiation and heat induced stress.

History

Glutathione reductase was first purified in 1955 at Yale by E. Racker. Racker also identified NADPH as the primary electron donor for the enzyme. Later groups confirmed the presence of FAD and the thiol group, and an initial mechanism was suggested for the mechanism in 1965. The initial (low resolution) structure of glutathione reductase was solved in 1977. This was quickly followed by a 3Å structure by Shulze et al. in 1978. Glutathione reductase has been studied exhaustively since these early experiments, and is subsequently one of the most well characterized enzymes to date.

Interactive pathway map

Interactive pathway can be found here: pathway map

Further reading

Category:Antioxidants Category:EC 1.8.1