Fura-2

**Fura-2**
Identifiers
CAS Number	113694-64-7
ChemSpider	51442
Jmol 3D model	Interactive image
PubChem	57054
	InChI InChI=1S/C29H27N3O14/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42) Key: YFHXZQPUBCBNIP-UHFFFAOYSA-N ; InChI=1/C29H27N3O14/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42) Key: YFHXZQPUBCBNIP-UHFFFAOYAO ;
	SMILES O=C(O)CN(c4ccc(cc4OCCOc3cc1c(oc(c1)c2ncc(o2)C(=O)O)cc3N(CC(=O)O)CC(=O)O)C)CC(=O)O ;
Properties
Chemical formula	C29H22N3O14K5
Molar mass	831.99 g/mol
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium.^[1] It was the first widely used dye for calcium imaging, and remains very popular. Fura-2 is excited at 340 nm and 380 nm of light, and the ratio of the emissions at those wavelengths is directly related to the amount of intracellular calcium. Regardless of the presence of calcium, Fura-2 emits at 510 nm of light. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels. The high photon yield of fura-2 allowed the first real time (video rate) measurements of calcium inside living cells in 1986.^[2] More recently, genetically-encoded calcium indicators based on spectral variants of the green fluorescent protein, such as Cameleons,^[3] have supplemented the use of Fura-2 and other small molecule dyes for calcium imaging, but Fura-2 remains faster.

References

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Fura-2

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Identifiers

CAS Number	113694-64-7^Y
ChemSpider	51442^Y
Jmol 3D model	Interactive image
PubChem	57054
InChI InChI=1S/C29H27N3O14/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42)^Y Key: YFHXZQPUBCBNIP-UHFFFAOYSA-N^Y InChI=1/C29H27N3O14/c1-15-2-3-17(31(11-24(33)34)12-25(35)36)20(6-15)43-4-5-44-21-7-16-8-22(28-30-10-23(46-28)29(41)42)45-19(16)9-18(21)32(13-26(37)38)14-27(39)40/h2-3,6-10H,4-5,11-14H2,1H3,(H,33,34)(H,35,36)(H,37,38)(H,39,40)(H,41,42) Key: YFHXZQPUBCBNIP-UHFFFAOYAO
SMILES O=C(O)CN(c4ccc(cc4OCCOc3cc1c(oc(c1)c2ncc(o2)C(=O)O)cc3N(CC(=O)O)CC(=O)O)C)CC(=O)O
Properties
Chemical formula	C₂₉H₂₂N₃O₁₄K₅
Molar mass	831.99 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Y verify (what is ^YN ?)
Infobox references