Human Protein Atlas
| 360px | |
|---|---|
| Content | |
| Description | The Human Protein Atlas portal is a publicly available database with millions of high-resolution images showing the spatial distribution of proteins in 44 different normal human tissues and 20 different cancer types, as well as 46 different human cell lines. |
| Organisms | Human |
| Contact | |
| Research center | KTH, UU, SciLifeLab, Sweden |
| Primary citation | Towards a knowledge-based Human Protein Atlas[1] |
| Access | |
| Website | www |
| Download URL | www |
| Tools | |
| Web | Advanced search, bulk retrieval/download |
| Miscellaneous | |
| Versioning | Yes |
| Data release frequency |
6 months |
| Version | 15 |
| Curation policy | Yes – manual |
| Bookmarkable entities |
Yes – both individual protein entries and searches |
The Human Protein Atlas (HPA) program is a scientific research program with the goal to explore the whole human proteome using an antibody-based approach. The program was started at the Royal Institute of Technology, Sweden, in 2003 and funded by the non-profit organization Knut and Alice Wallenberg Foundation (KAW).
The project has a gene-centric approach with the effort to map and characterize a representative protein for each protein-coding human gene (approximately 20,000 genes). Antibodies, both in-house produced and external (commercial and from collaborators), are validated in the HPA workflow and used for protein characterization. The data is released annually in a publicly available information database portal.[1]
The database includes protein expression profiles from 44 different normal and 20 different cancer tissues, 46 cell lines, subcellular localization and transcript expression levels. The database is searchable for a specific gene or protein. Functionalities added to the The Human Protein Atlas in later versions allow for combined searches, e.g. to find all proteins expressed in a certain organ or tissue or proteins differentially expressed in a specific tumor type.
Contents
History
The HPA program was started in 2003 and funded by the non-profit organization Knut and Alice Wallenberg Foundation (KAW). The main site of the project is the Royal Institute of Technology (KTH), School of Biotechnology (Stockholm, Sweden). Professor Mathias Uhlén is the coordinator of the program.
The research underpinning the start of the exploration of the whole human proteome in the Human Protein Atlas program was carried out in the late 1990s and early 2000s. A pilot study employing an affinity proteomics strategy using affinity-purified antibodies raised against recombinant human protein fragments was carried out for a chromosome-wide protein profiling of chromosome 21.[2] Other projects were also carried out to establish processes for parallel and automated affinity purification of mono-specific antibodies and their validation.,[3][4]
Research
Antibodies and antigens, produced in the HPA workflow, are used in research projects to study potential biomarkers in various diseases, such as breast cancer, prostate cancer, colon cancer, diabetes, autoimmune diseases, ovarian cancer and renal failure.[5][6][7][8][9][10]
In close collaboration to the HPA, a rodent brain atlas is also being built using the HPA-validated antibodies. Protein distribution is investigated in the nervous system of the rodent brain.[11]
Collaborations
The HPA program is participating in 9 EU research projects ENGAGE, PROSPECTS, BIO_NMD, AFFINOMICS, CAGEKID, EURATRANS, ITFoM, DIRECT and PRIMES.
References
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