MCM6

Minichromosome maintenance complex component 6
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2KLQ, 2LE8
Identifiers
Symbols	MCM6 ; MCG40308; Mis5; P105MCM
External IDs	OMIM: 601806 MGI: 1298227 HomoloGene: 4322 GeneCards: MCM6 Gene
EC number	3.6.4.12
Gene ontology Molecular function • single-stranded DNA binding • ATP-dependent DNA helicase activity • protein binding • ATP binding • identical protein binding Cellular component • nucleus • nucleoplasm • MCM complex Biological process • G1/S transition of mitotic cell cycle • mitotic cell cycle • DNA replication • DNA unwinding involved in DNA replication • DNA replication initiation • DNA strand elongation involved in DNA replication Sources: Amigo / QuickGO
RNA expression pattern
File:PBB GE MCM6 201930 at tn.png
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4175	17219
Ensembl	ENSG00000076003	ENSMUSG00000026355
UniProt	Q14566	P97311
RefSeq (mRNA)	NM_005915	NM_008567
RefSeq (protein)	NP_005906	NP_032593
Location (UCSC)	Chr 2: 135.84 – 135.88 Mb	Chr 1: 128.33 – 128.36 Mb
PubMed search	[1]	[2]

DNA replication licensing factor MCM6 is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication.

Function

The MCM complex consisting of MCM6 (this protein) and MCM2, 4 and 7 possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Mcm 6 has recently been shown to interact strongly Cdt1 at defined residues, by mutating these target residues Wei et al. observed lack of Cdt1 recruitment of Mcm2-7 to the pre-RC.

Gene

The MCM6 gene, MCM6, is expressed at very high level. MCM6 contains 18 introns. There are 2 non overlapping alternative last exons. The transcripts appear to differ by truncation of the 3' end, presence or absence of 2 cassette exons, common exons with different boundaries.

MCM6 produces, by alternative splicing, 3 different transcripts, all with introns, putatively encoding 3 different protein isoforms.

MCM6 contains two of the regulatory regions for LCT, the gene encoding the protein lactase, located in two of the MCM6 introns, approximately 14 kb (-13910) and 22 kb (-22018) upstream of LCT. The (-13910) region, in particular, has been shown to function in vitro as an enhancer element capable of differentially activating transcription of LCT promoter.

Mutations in these regions are associated with lactose tolerance into adult life.

Interactions

MCM6 has been shown to interact with:

• CDC45-related protein,
• MCM2,
• MCM4,
• MCM7,
• ORC1L,
• ORC2L,
• ORC4L, and
• Replication protein A1.

See also

• Mini Chromosome Maintenance