Wayson stain

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The Wayson stain is a basic fuchsin-methylene blue, ethyl alcohol-phenol microscopic staining procedure. It was originally a modified methylene blue stain used for diagnosing bubonic plague.[1] With this stain, Yersinia pestis appears purple with a characteristic safety-pin appearance,[2] which is due to the presence of a central vacuole.

Wayson stain is used along with the Giemsa and Wright's stains to rapidly detect potential biowarfare attacks[3] It has also been investigated as a possible cheaper and faster way to detect melioidosis.[4] It is a useful alternative to the Gram or Loeffler’s Methylene Blue stains, especially for detecting Yersinia enterocolitica which is often found in contaminated food[5]

References

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  3. Medscape.com Clues to the Specific Etiologic Agent Used in a Biological Attack
  4. Lua error in package.lua at line 80: module 'strict' not found.
  5. CLP Magazine Aug 2001

External links