GRB7

Growth factor receptor-bound protein 7
250px PDB rendering based on 1mw4.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1MW4, 1WGR, 2L4K, 2QMS, 3PQZ
Identifiers
Symbol	GRB7
External IDs	OMIM: 601522 MGI: 102683 HomoloGene: 3881 ChEMBL: 1649051 GeneCards: GRB7 Gene
Gene ontology Molecular function • RNA binding • SH3/SH2 adaptor activity • protein binding • protein kinase binding • phosphatidylinositol binding • identical protein binding Cellular component • cytosol • plasma membrane • focal adhesion • cytoplasmic stress granule • cell projection Biological process • epidermal growth factor receptor signaling pathway • blood coagulation • positive regulation of signal transduction • negative regulation of translation • positive regulation of cell migration • stress granule assembly • leukocyte migration Sources: Amigo / QuickGO
RNA expression pattern
File:PBB GE GRB7 210761 s at tn.png
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	2886	14786
Ensembl	ENSG00000141738	ENSMUSG00000019312
UniProt	Q14451	Q03160
RefSeq (mRNA)	NM_001030002	NM_010346
RefSeq (protein)	NP_001025173	NP_034476
Location (UCSC)	Chr 17: 39.74 – 39.75 Mb	Chr 11: 98.45 – 98.46 Mb
PubMed search	[1]	[2]

Growth factor receptor-bound protein 7, also known as GRB7, is a protein that in humans is encoded by the GRB7 gene.

Function

The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date.

Clinical significance

GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular direct link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene.

These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7 thought to be involved in migration, is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.

Interactions

GRB7 has been shown to interact with:

• EPH receptor B1,
• Insulin receptor,
• PTK2,
• RET proto-oncogene, and
• Rnd1

Model organisms

Model organisms have been used in the study of GRB7 function. A conditional knockout mouse line called Grb7^{tm1b(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Additional screens performed: - In-depth immunological phenotyping

Grb7 knockout mouse phenotype

Characteristic	Phenotype
All data available at.
Peripheral blood leukocytes 6 Weeks	Normal
Haematology 6 Weeks	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Trichuris Challenge	Normal

External links

• GRB7 Adaptor Protein at the US National Library of Medicine Medical Subject Headings (MeSH)